Our antibody labeling collection offers various ways for primary antibody labeling. The antibody labeling chemistry we provide involves either free amine groups or free sulfhydryl groups. Direct antibody conjugation enables the labeled antibody to be used in further immunoassay analyses. We provide different kinds of conjugation detection methods as follows:
- Biotin/Streptavidin: Avidin, Biotin, Streptavidin
- Enzyme: AP, Glucose oxidase, HRP
- Fluorophore: AMCA, FITC, PE, PerCP, Rhodamine
- Tandem: Texas Red or Atto594 combined with PE
- Lanthanide: Europium
With the variety of labels we provide, conjugated antibodies may further be applied to the immunoassays listed in the table below:
|
| Immunoassay |
Labels |
| Western Blot |
Enzyme (usually HRP or AP) |
| ELISA |
Enzyme, Biotin/Streptavidin |
| Immunofluorescence |
Fluorescent dyes |
| Immunohistochemistry |
Enzyme, Biotin/Streptavidin |
| Fluorescence Technology |
Fluorescent dyes, Tandem dyes, Lanthanide |
|
| |
| Table of Fluorochromes |
| Color |
Fluorochromes |
Catalog ID |
Maximal Absorbance (nm) |
Maximal Emission (nm) |
| |
AMCA |
KA1559 |
346 |
442 |
| |
DyLight® 350 |
|
353 |
432 |
| |
Atto390 |
KA1560 |
390 |
479 |
| |
Atto425 |
KA1561 |
436 |
484 |
| |
Atto465 |
KA1562 |
453 |
508 |
| |
Atto488 |
KA1563 |
501 |
532 |
| |
DyLight® 488 |
|
493 |
518 |
| |
R-Phycoerythrin (R-PE) |
KA0012, KA0013, KA1553 |
480/565 |
578 |
| |
Fluorescein |
KA0009, KA1571 |
485 |
535 |
| |
PerCP |
KA1552 |
490 |
675 |
| |
Atto532 |
KA1564 |
532 |
553 |
| |
Cyanine Dye 3 (Cy3) |
|
550 |
570 |
| |
FluoProbes547H |
KA1592 |
557 |
572 |
| |
Rhodamine |
KA1572 |
544 |
576 |
| |
B-Phycoerythrin (B-PE) |
KA0007, KA0008, KA1574 |
546/565 |
575 |
| |
HiLyte FluorTM 555 |
KA0010 |
550 |
566 |
| |
DyLight®550 |
|
562 |
576 |
| |
Atto565 |
KA1565 |
563 |
592 |
| |
Cyanine Dye 3.5 (Cy3.5) |
|
581 |
596 |
| |
Texas Red |
KA1573 |
595 |
615 |
| |
FluoProbes594 |
KA1591 |
591 |
616 |
| |
DyLight®594 |
|
593 |
618 |
| |
Atto590 |
KA1566 |
600 |
627 |
| |
Atto594 |
KA1567 |
601 |
627 |
| |
Atto633 |
KA1568 |
629 |
657 |
| |
DyLight®633 |
|
638 |
658 |
| |
Atto637 |
KA1583 |
630 |
659 |
| |
Cyanine Dye 5 (Cy5) |
|
643 |
667 |
| |
HiLyte FluorTM 647 |
KA0011 |
649 |
674 |
| |
Allophycocyanin (APC) |
KA0005, KA0006, KA1554 |
650 |
660 |
| |
DyLight®650 |
|
652 |
672 |
| |
FluoProbes647H |
KA1593 |
653 |
675 |
| |
Atto655 |
KA1584 |
663 |
684 |
| |
Cyanine Dye 5.5 (Cy5.5) |
|
675 |
694 |
| |
FluoProbes682 |
KA1594 |
690 |
709 |
| |
Atto680 |
KA1569 |
670 |
710 |
| |
DyLight®680 |
|
652 |
672 |
| |
Atto700 |
KA1570 |
700 |
725 |
| |
FluoProbes752 |
KA1590 |
748 |
772 |
| |
Cyanine Dye 7 (Cy7) |
|
747 |
776 |
| |
DyLight® 755 |
|
754 |
778 |
| |
DyLight®800 |
|
770 |
794 |
|
