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Our antibody labeling collection offers various ways for primary antibody labeling. The antibody labeling chemistry we provide involves either free amine groups or free sulfhydryl groups. Direct antibody conjugation enables the labeled antibody to be used in further immunoassay analyses. We provide different kinds of conjugation detection methods as follows:

  • Biotin/Streptavidin: Avidin, Biotin, Streptavidin
  • Enzyme: AP, Glucose oxidase, HRP
  • Fluorophore: AMCA, FITC, PE, PerCP, Rhodamine
  • Tandem: Texas Red or Atto594 combined with PE
  • Lanthanide: Europium

With the variety of labels we provide, conjugated antibodies may further be applied to the immunoassays listed in the table below:
Immunoassay Labels
Western Blot Enzyme (usually  HRP or AP)
ELISA Enzyme, Biotin/Streptavidin
Immunofluorescence Fluorescent dyes
Immunohistochemistry Enzyme, Biotin/Streptavidin
Fluorescence Technology Fluorescent dyes, Tandem dyes, Lanthanide
 
Table of Fluorochromes
Color Fluorochromes Catalog ID Maximal Absorbance (nm) Maximal Emission (nm)
  AMCA KA1559 346 442
  DyLight® 350   353 432
  Atto390 KA1560 390 479
  Atto425 KA1561 436 484
  Atto465 KA1562 453 508
  Atto488 KA1563 501 532
  DyLight® 488   493 518
  R-Phycoerythrin (R-PE) KA0012, KA0013, KA1553 480/565 578
  Fluorescein KA0009, KA1571 485 535
  PerCP KA1552 490 675
  Atto532 KA1564 532 553
  Cyanine Dye 3 (Cy3)   550 570
  FluoProbes547H KA1592 557 572
  Rhodamine KA1572 544 576
  B-Phycoerythrin (B-PE) KA0007, KA0008, KA1574 546/565 575
  HiLyte FluorTM 555 KA0010 550 566
  DyLight®550   562 576
  Atto565 KA1565 563 592
  Cyanine Dye 3.5 (Cy3.5)   581 596
  Texas Red KA1573 595 615
  FluoProbes594 KA1591 591 616
  DyLight®594   593 618
  Atto590 KA1566 600 627
  Atto594 KA1567 601 627
  Atto633 KA1568 629 657
  DyLight®633   638 658
  Atto637 KA1583 630 659
  Cyanine Dye 5 (Cy5)   643 667
  HiLyte FluorTM 647 KA0011 649 674
  Allophycocyanin (APC) KA0005, KA0006, KA1554 650 660
  DyLight®650   652 672
  FluoProbes647H KA1593 653 675
  Atto655 KA1584 663 684
  Cyanine Dye 5.5 (Cy5.5)   675 694
  FluoProbes682 KA1594 690 709
  Atto680 KA1569 670 710
  DyLight®680   652 672
  Atto700 KA1570 700 725
  FluoProbes752 KA1590 748 772
  Cyanine Dye 7 (Cy7)   747 776
  DyLight® 755   754 778
  DyLight®800   770 794

 

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