IFNG (Human) Recombinant Protein
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Functional Study
The ED50 for this effect is <1 ng/mL, measured by the induction of cytotoxicity in HT29 cells.
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Specifications
Product Description
Human IFNG recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 17.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.05 EU/ug
Activity
The ED50 for this effect is < 1 ng/mL, measured by the induction of cytotoxicity in HT29 cells. The specific activity of recombinant human IFN gamma is approximately > 1 x 107 IU/mg, which is calibrated against the human IFN Gamma WHO Reference Material (NIBSC code: 87/586).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Regulatory Status
GMP grade
Storage Buffer
Lyophilized from PBS, pH 8.0.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <1 ng/mL, measured by the induction of cytotoxicity in HT29 cells.SDS-PAGE
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Gene Info — IFNG
Entrez GeneID
3458Gene Name
IFNG
Gene Alias
IFG, IFI
Gene Description
interferon, gamma
Gene Ontology
HyperlinkGene Summary
Interferon-gamma (IFNG), or type II interferon, is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNG in the immune system stems in part from its ability to inhibit viral replication directly, but most importantly derives from its immunostimulatory and immunomodulatory effects. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 (MIM 186940) and CD8 (see MIM 186910) cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops (Schoenborn and Wilson, 2007 [PubMed 17981204]).[supplied by OMIM
Other Designations
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Interactomes
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Pathways
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Diseases
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