IL7 (Human) Recombinant Protein
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Functional Study
The ED50 for this effect is <0.8 ng/mL, measured in a cell proliferation assay using PHAactivated human peripheral blood lymphocytes (PBMC).
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Specifications
Product Description
Human IL7 recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 18.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.05 EU/ug
Activity
The ED50 for this effect is < 0.8 ng/mL, measured by the induction of PHA-activated human PBMCs proliferation. The specific activity of recombinant human IL-7 is > 1 x 108 units/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Regulatory Status
GMP grade
Storage Buffer
Lyophilized from PBS, pH 8.0.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <0.8 ng/mL, measured in a cell proliferation assay using PHAactivated human peripheral blood lymphocytes (PBMC).SDS-PAGE
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Gene Info — IL7
Entrez GeneID
3574Gene Name
IL7
Gene Alias
IL-7
Gene Description
interleukin 7
Omim ID
146660Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. [provided by RefSeq
Other Designations
IL7 nirs variant 6
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Interactomes
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Pathways
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Diseases
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