Stable Cell Line Antibody Production

Abnova is specialized in rapid and efficient establishment of stable cell lines that can constitutively express excellent quality of rabbit, mouse, or human monoclonal antibody. High efficient expression constructs are transfected and stably integrated into the mammalian 293 or CHO-S cell genome. After multiple post-transfection passages in selection media, high expression clones from stably transfected population are then isolated and verified for IgG expression by SDS-PAGE Coomassie Blue staining and ELISA. Subsequent development and optimized growth of these positive clones will be promoted in serum-free suspension environment. Within 2-4 months, top three expression cell lines with three vials each will be ready for delivery. Service for antibody production following generation of the stable cell lines at gram quantities is also available. The actual turnaround time for scale-up antibody production and purification may vary for each cell line.