ARPC5 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with p16 ARC recombinant monoclonal antibody (Cat # RAB02446).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using p16 ARC recombinant monoclonal antibody (Cat # RAB02446). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.43). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ARPC5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human p16 ARC.
Theoretical MW (kDa)
16
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of p16 ARC protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with p16 ARC recombinant monoclonal antibody (Cat # RAB02446).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using p16 ARC recombinant monoclonal antibody (Cat # RAB02446). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.43). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — ARPC5
Entrez GeneID
10092Protein Accession#
O15511Gene Name
ARPC5
Gene Alias
ARC16, MGC88523, dJ127C7.3, p16-Arc
Gene Description
actin related protein 2/3 complex, subunit 5, 16kDa
Omim ID
604227Gene Ontology
HyperlinkGene Summary
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. [provided by RefSeq
Other Designations
Arp2/3 protein complex subunit p16|OTTHUMP00000033266|actin related protein 2/3 complex subunit 5|actin related protein 2/3 complex, subunit 5 (16 kD)
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Interactome
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Pathway
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