ALCAM recombinant monoclonal antibody, clone R03-2E5
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More Files
- More Functions
Western Blot
Western blot analysis of CD166 in rat Brain, C6 lysates using human CD166 epsilon recombinant monoclonal antibody, clone R03-2E5 (Cat # RAB01527).
Immunocytochemistry
Immunocytochemistry analysis of CD166 (green) in SKOV-3 using human CD166 recombinant monoclonal antibody, clone R03-2E5 (Cat # RAB01527), and DAPI(blue).
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human CD166.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD166
Theoretical MW (kDa)
Calculated MW: 65 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CD166 in rat Brain, C6 lysates using human CD166 epsilon recombinant monoclonal antibody, clone R03-2E5 (Cat # RAB01527).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of CD166 (green) in SKOV-3 using human CD166 recombinant monoclonal antibody, clone R03-2E5 (Cat # RAB01527), and DAPI(blue).Immunoprecipitation
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Gene Info — ALCAM
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Interactomes
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Pathways
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Diseases
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