Products

Western Blot (Tissue lysate)

Western blot using UBE2N polyclonal antibody (Cat # PAB11286) shows detection of UBE2N protein inhuman small intestine lysate (Lane 1), but not in mouse thymus lysate (Lane 2).
The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein.
The identity of minor reactive bands is unknown, but could represent E2 complexes.
Each lane contains approximately 20 ug of lysate.
Primary antibody was used at a 1 : 500 dilution.
The membrane was washed and reacted with a 1 : 10,000 dilution of Alexa Fluor™ 680 conjugated Rb-a-Goat IgG.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

7334

Protein Accession#

NP_003339;P61088

Gene Name

UBE2N

Gene Alias

MGC131857, MGC8489, UBC13, UbcH-ben

Gene Description

ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)

Omim ID

603679

Gene Ontology

Hyperlink

Gene Summary

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq

Other Designations

bendless-like ubiquitin conjugating enzyme|ubiquitin carrier protein N|ubiquitin-conjugating enzyme E2N|ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)|ubiquitin-protein ligase N|yeast UBC13 homolog