One of the most convenient and promising eukaryotic cell-free translation systems is based on wheat embryos that store all of the components of translation in a concentrated dried state, ready for protein synthesis as soon as germination starts. Conventional wheat germ extracts contain the RNA N-glycosidase tritin and other inhibitors of translation such as thionin, ribonucleases, deoxyribonucleases, and proteases. These inhibitors originate from the endosperm. Extensive washing of wheat embryos to eliminate endosperm contaminants has resulted in extracts with a high degree of stability and activity. By using mRNA having 5′-cap and a poly(A)-tail with this extract, the translation reaction yields mg quantities of active proteins per ml of reaction volume.

Features Concentrated Translational Mixture Free of Translation Inhibitors High Stability and Activity Suitable for Eukaryotic Cell-Free Expression
Unwashed Wheat Embyro Washed Wheat Embyro